I found the link to a commercial product by Evrogen to normalise cDNA samples for gene discovery projects here:
http://www.evrogen.com/technologies/normalization.shtml
The most up-to-date reference seems to be this Curr Protoc Mol Biol. paper by Bogdanova et al.:
http://www.ncbi.nlm.nih.gov/pubmed/20373503
They claim their method is compatible with nextgen sequencing platforms:
cDNA normalization using duplex-specific nuclease (DSN) is a highly
efficient approach that can be applied for normalization of
full-length-enriched cDNA (Zhulidov et al., 2004; Zhulidov et al.,
2005). The resulting cDNA contains equalized abundance of different
transcripts and can be used for construction of cDNA libraries and for
direct sequencing, including high-throughput sequencing on the next
generation sequencing platforms (Roche/454, ABI/SOLiD or
Illumina/Solexa).
Has anybody successfully used it for next-gen gene discovery projects?
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