The fastest will change as time passes and better technologies are developed.
I think the fastest method existing at the moment is Shotgun Mutagenesis (provided by Integral Molecular Inc).
This does not employ any new method of doing that. They just provide a set of plasmids, that has all the possible mutations. The set itself is generated by automated DNA synthesis.
So if you don't have a DNA synthesizer with you then simply order the kit from the company.
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