Wednesday, 25 November 2009

lab techniques - Can I image Coomassie and GFP in gels at the same time with a fluorescence scanner?

I'm working with a GFP-tagged protein and am routinely using a fluorescence imager (GE Typhoon) and a standard optical scanner to capture fluorescent and absorption images, respectively, of my SDS-PAGE gels. The Typhoon supports multiple channels, so is there some way that I can scan for the two on the same device (GFP + total protein)? I assume I can't do absorption on a fluorescence scanner..



I could manually register the two images, but it would be laborious as they are at different resolutions, slightly different rotations, and the gel can stretch ever-so-slightly when placing it on the platens.

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