As Thomas pointed out, there is a maximum ramp rate which you can set up on the thermocycler. Its value can go from 0% to 100%. I can then assume that a 100% ramp rate will corespond to the maximum ramp rate of 4.25°C/s for the heating block or 5°C/s for the sample. which means that a ramp rate of ~2.4%. Subsequently, I set up my reaction at a 2% ramp rate, as I started a timer at the time the ramp cycle started. To my surprise, the temperature was cooling down too slowly. Thus, I empirically found out that a ramp rate of 5% corresponds to ~0.1°C/s cooling speed.
I wished there was more precise way to calculate it. Perhaps the slope of the line changed at different temperature intervals. It would be great if I can talk to the AB people so that they could justify my finding.
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