Wednesday, 30 May 2007

biochemistry - How can I measure bacterial alkaline phosphatase activity?

I want to measure alkaline phosphatase activity using PNPP in my mutant bacteria strains, but all the protocols I found involve purification of the phosphatase (which I have no need of).



Does anyone know a good protocol which involves using only the supernatant, without purification of the enzyme? What is the preferred buffer for the reaction?

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