I would like to do a quick freeze on pancreas from mice. I want to then make sections (30 µm thick). The idea is to preserve a fluorescent staining done in vivo.
I do not know if I should place my fresh tissue directly into liquid nitrogen, then embed the tissue in OCT, and then freeze everything in cold isopentane?
Or do I have to embed the fresh tissue in OCT first, prior to putting everything in nitrogen?
Or is there another protocol to do quick freezing in order to do sectioning?
No comments:
Post a Comment