Sunday, 13 August 2006

gel electrophoresis - How often can you reuse coomassie stain?

Since there's a lot of methanol in Coomassie stain, a significant amount would probably evaporate off each time you microwave it. Therefore, it'd be probably a good idea not to reuse it because (1) you're losing methanol, and (2) the concentration of everything else is thrown off.



Why do you boil the stain? I just pour mine at RT right on the gel and it works fine.

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